TRYPSINOGEN AND CHYMOTRYPSINOGEN AS HOMOLOGOUS PROTEINS
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چکیده
منابع مشابه
Inactivation of bovine trypsinogen and chymotrypsinogen by diisopropylphosphorofluoridate.
Diisopropylphosphorofluoridate reacts with trypsinogen and chymotrypsinogen and inhibits the potential activity of both zymogens. The reactions follow pseudo first-order kinetics and proceed approximately four orders of magnitude slower than diisopropylphosphorylation of the corresponding enzymes. Correlation of initial rates of inactivation with incorporation of the reagent indicates that zymo...
متن کاملCalorimetric characterization of ligand binding on trypsinogen and chymotrypsinogen A, with evidence for two binding sites on alpha-chymotrypsin.
Heat is evolved when solutions of trypsinogen or chymotrypsinogen A are mixed with solutions of the amide or methyl ester derivatives of NQ-p-toluenesulfonyl-L-arginine, appropriate corrections being made for heats of reactant dilution. Exothermic reaction was also observed when the two ligands were mixed with tr-chymotrypsin; all measurements were made at 25”, pH 5.0. The dependence of apparen...
متن کاملCalorimetric Characterization of Ligand Binding on Trypsinogen and Chymotrypsinogen A, with Evidence for Two Binding Sites on &hymotrypsin*
Heat is evolved when solutions of trypsinogen or chymotrypsinogen A are mixed with solutions of the amide or methyl ester derivatives of NQ-p-toluenesulfonyl-L-arginine, appropriate corrections being made for heats of reactant dilution. Exothermic reaction was also observed when the two ligands were mixed with tr-chymotrypsin; all measurements were made at 25”, pH 5.0. The dependence of apparen...
متن کاملRegulation of concentrations of mRNA for amylase, trypsinogen I and chymotrypsinogen B in rat pancreas by secretagogues.
Regulation of pancreatic gene expression by the secretory stimulants cholecystokinin-pancreozymin, caerulein and pilocarpine was studied in the rat, by using cloned cDNA probes to quantify concentrations of specific mRNAs (amylase, trypsinogen I and chymotrypsinogen B). It is concluded that long-term pancreatic stimulation results in pre-translational regulation of secretory-protein gene expres...
متن کاملQuantifying homologous proteins and proteoforms
Many proteoforms - arising from alternative splicing, post-translational modifications (PTMs), or paralogous genes - have distinct biological functions, such as histone PTM proteoforms. However, their quantification by existing bottom-up mass-spectrometry (MS) methods is undermined by peptide-specific biases. To avoid these biases, we developed and implemented a first-principles model (HIquant)...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1964
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.52.4.884